Analysis of Spatial Learning in Honeybee Foragers Using the Food Search Box Assay

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dc.contributor.advisorZayed, Amro
dc.contributor.authorMorrison, Bandele Mojoyinola Abike
dc.date.accessioned2020-11-13T13:55:47Z
dc.date.available2020-11-13T13:55:47Z
dc.date.copyright2020-09
dc.date.issued2020-11-13
dc.date.updated2020-11-13T13:55:47Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractGiven the under-representation of genetic analyses of spatial learning in honeybee literature, I designed an experiment to investigate gene expression profiles associated with spatial learning. The experimental design involved using the Food Search Box (FSB) assay to assess learning in Single Drone-Inseminated (SDI) foragers, then collecting the bees for RNA-seq analysis to generate and compare full-transcriptome gene expression profiles for five different stages of learning. From the first to the third learning trial, the SDI foragers did not decrease the number of mistakes they made in a significant way: they did not switch from chance to non-chance searching behaviour. In addition, during the memory recall stage, the bees made significantly more errors than chance, suggesting they did not remember their focal flower. Landmarks cues, inter-trial interval, motivation level for sucrose and genetics may each play a role in the ability of SDI foragers to learn vs. foragers from naturally mated queens.
dc.identifier.urihttp://hdl.handle.net/10315/37950
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectAnimal behavior
dc.subject.keywordsHoneybee
dc.subject.keywordsApis mellifera
dc.subject.keywordsLearning
dc.subject.keywordsMemory
dc.subject.keywordsSpatial learning
dc.subject.keywordsGene expression
dc.subject.keywordsTranscriptome
dc.subject.keywordsSingle drone insemination
dc.subject.keywordsForager
dc.subject.keywordsFood search box
dc.subject.keywordsLearning assay
dc.subject.keywordsLearning protocol
dc.subject.keywordsWin shift
dc.subject.keywordsWin stay
dc.subject.keywordsNavigation
dc.subject.keywordsWaggle dance
dc.subject.keywordsDance communication
dc.subject.keywordsDance language
dc.titleAnalysis of Spatial Learning in Honeybee Foragers Using the Food Search Box Assay
dc.typeElectronic Thesis or Dissertation

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