Characterization of RBL2 in Muscle Stem Cell Fate Decisions

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dc.contributor.advisorScime, Anthony
dc.contributor.authorCampagna, Lucas
dc.date.accessioned2023-12-08T14:51:04Z
dc.date.available2023-12-08T14:51:04Z
dc.date.issued2023-12-08
dc.date.updated2023-12-08T14:51:03Z
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractSkeletal muscle stem cells (MuSCs) regenerate muscle upon injury. Here, they receive activation signals from their environment and make a fate decision to make further copies (self-renew) or commit to becoming muscle (differentiate). Dysfunctional MuSC fate decisions, lead to poor muscle quality. Accordingly, retinoblastoma-like protein 2 (Rbl2) was characterized in MuSC fate decisions. Targeted deletion of Rbl2 in muscle progenitors impaired myotube formation, demonstrating its potential requirement for MuSC function. Rbl2 was analyzed during MuSC fate decisions by immunostaining cultured myofibers with Pax7 and MyoD. Rbl2 was expressed in the nucleus of only a fraction of committed MuSCs. Surprisingly, during a differentiation time course, primary myogenic progenitor cells expressed Rbl2 in the mitochondria. The Rbl2 expression in the mitochondria of differentiating MuSCs was confirmed in vivo by immunohistochemistry. These results provide novel insights for the localization of Rbl2 in MuSC fate decisions and the mechanisms driving differentiation.
dc.identifier.urihttps://hdl.handle.net/10315/41791
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectCellular biology
dc.subjectMolecular biology
dc.subjectPhysiology
dc.subject.keywordsMuscle
dc.subject.keywordsMuscle stem cell
dc.subject.keywordsMuSC
dc.subject.keywordsStem cell
dc.subject.keywordsFate decisions
dc.subject.keywordsRetinoblastoma
dc.subject.keywordsRetinoblastoma-like protein 2
dc.subject.keywordsRBL2
dc.subject.keywordsDifferentiation
dc.subject.keywordsMuscle regeneration
dc.subject.keywordsMitochondria
dc.titleCharacterization of RBL2 in Muscle Stem Cell Fate Decisions
dc.typeElectronic Thesis or Dissertation

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