Regulation of MMP9 by MEF2A and STAT3 in Neonatal Rat Ventricular Cardiomyocytes

Simple item page
dc.contributor.advisorMcDermott, John Charles
dc.contributor.authorBrar, Gurnoor Kaur
dc.date.accessioned2023-08-04T15:05:55Z
dc.date.available2023-08-04T15:05:55Z
dc.date.issued2023-08-04
dc.date.updated2023-08-04T15:05:55Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThe myocyte enhancer factor-2 (MEF2) transcription factor is critical for defining cardiac-specific gene expression programs that have fundamental roles in differentiation and development. This thesis explored the effects of a novel protein:protein interaction between the MEF2A and signal transducer and activator of transcription-3 (STAT3) on matrix metalloproteinase (MMP9) transcriptional regulation in neonatal rat ventricular cardiomyocytes (NRVMs). Immunoprecipitation experiments confirmed a robust interaction between endogenous MEF2A and STAT3 within the NRVMs. SiRNA-mediated gene silencing and pharmacological inhibition revealed that MMP9 promoter activity, mRNA, and protein expression are significantly enhanced upon STAT3 depletion/inhibition and there is a marginal decrease in MMP9 mRNA upon MEF2A depletion. The foundational work in this thesis sheds light on the MMP9 gene transcriptional regulation by both MEF2A and STAT3, having implications for cardiac physiology and pathology associated with MMP9.
dc.identifier.urihttps://hdl.handle.net/10315/41298
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subject.keywordsMEF2A
dc.subject.keywordsMMP9
dc.subject.keywordsSTAT3
dc.subject.keywordsCardiomyocytes
dc.subject.keywordsCardiac Physiology
dc.titleRegulation of MMP9 by MEF2A and STAT3 in Neonatal Rat Ventricular Cardiomyocytes
dc.typeElectronic Thesis or Dissertation

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Brar_Gurnoor_K_2023_Masters.pdf
Size:
1.8 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
license.txt
Size:
1.87 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
YorkU_ETDlicense.txt
Size:
3.39 KB
Format:
Plain Text
Description:
Download

Collections

Biology

All items in the YorkSpace institutional repository are protected by copyright, with all rights reserved except where explicitly noted.