Optofluidic Add-on Devices for Light Sheet Fluorescence Microscopy of Caenorhabditis Elegans with Conventional Wide-Field Microscopes

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dc.contributor.advisorRezai, Pouya
dc.contributor.advisorTabatabaei, Nima
dc.contributor.authorBehrouzi, Mehran
dc.date.accessioned2022-08-08T15:46:43Z
dc.date.available2022-08-08T15:46:43Z
dc.date.copyright2022-02-25
dc.date.issued2022-08-08
dc.date.updated2022-08-08T15:46:42Z
dc.degree.disciplineMechanical Engineering
dc.degree.levelMaster's
dc.degree.nameMASc - Master of Applied Science
dc.description.abstractThis research reports on the development of low-cost and compact optofluidic add-on devices for converting a conventional wide-field microscope to a light sheet fluorescence microscope for immobilization-free, rapid and continuous imaging of the C. elegans model organism. The developed Polydimethylsiloxane (PDMS)-based optofluidic devices consist of an integrated PDMS cylindrical lens for on-chip light-sheet generation and a microfluidics channel for flow-based translation of samples through the light sheet. Validation experiments on several strains of C. elegans demonstrated the ability of the developed imaging platform in volumetric imaging of the fluorescence expressions of entire worms in a few seconds, at the single-neuron resolution, and with high contrast. Also, results of the primary neurodegeneration study demonstrated the ability of the platform on quantifying the fluorescent expressions of the worms and detecting different neurotoxin-induced phenotypes.
dc.identifier.urihttp://hdl.handle.net/10315/39592
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiomedical engineering
dc.subject.keywordsLight sheet fluorescence microscopy
dc.subject.keywordsSelective plane illumination microscopy
dc.subject.keywordsC. elegans
dc.subject.keywordsMicrofluidics
dc.subject.keywordsOptofluidic
dc.subject.keywordsOrganism-on-a-chip
dc.subject.keywordsBiomedical imaging
dc.subject.keywordsFluorescent imaging
dc.titleOptofluidic Add-on Devices for Light Sheet Fluorescence Microscopy of Caenorhabditis Elegans with Conventional Wide-Field Microscopes
dc.typeElectronic Thesis or Dissertation

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