Effect of Saline and Non-Specific Insulin Binding on the Phase Behavior of Poly (Ethylene Glycol)-Grafted Phosphoethanolamine-Succinyl Model Membranes

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dc.contributor.advisorTsoukanova, Valeria
dc.creatorShahid, Muhammad Naeem
dc.date.accessioned2014-07-10T15:28:39Z
dc.date.available2014-07-10T15:28:39Z
dc.date.copyright2013-09-13
dc.date.issued2014-07-09
dc.date.updated2014-07-09T15:56:03Z
dc.degree.disciplineChemistry
dc.degree.levelDoctoral
dc.degree.namePhD - Doctor of Philosophy
dc.description.abstractPoly (ethylene glycol)-grafted membrane-mimetic surfaces bearing negatively charged phospholipid headgroups have gained significant attention due to their promising contributions in numerous biomedical applications. The conformational properties of PEG chains have been mainly studied at the air/water interface, which does not elucidate much about its behavior at the physiological pH ~ 7.4. In this contribution, binary mixtures of a phosphoethanolamine-Succinyl bearing C16 aliphatic chains, DPPE-Succinyl, and a PEG-phospholipid conjugate bearing a PEG chain of 2000 Da, DPPE-PEG2000, have been used as ideal models of bio-nonfouling membrane-mimetic surfaces. The effect of PBS with pH ~7.4 as well as each of its individual constituents including Na2HPO4, KCl, KH2PO4, and NaCl on the biophysical properties of model membrane was examined. Our findings suggest that saline and each of its individual constituents play a pivotal role in the phase and conformational behavior of PEG-grafted membrane models. Insulin as a model protein was then selected to further investigate the effect of phase and conformation behavior of PEG-grafted membrane models on protein/membrane interactions. The insulin/membrane interactions were quantified in terms of monolayer area expansion, ΔA, penetration area, Ap, as well as protein binding degree, χp. To the best of our knowledge, this study provides the first insight into mechanistic aspects of protein interactions with model negatively charged PEG-grafted membranes. This knowledge, may aid in understanding the in-vivo performance of advanced targeted therapeutic carriers.en_US
dc.identifier.urihttp://hdl.handle.net/10315/27557
dc.language.isoenen_US
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectChemistryen_US
dc.subject.keywordsInsulin Conformationen_US
dc.subject.keywordsPhase Behavioren_US
dc.subject.keywordsSalineen_US
dc.subject.keywordsPBSen_US
dc.subject.keywordsNon-specific Insulin Bindingen_US
dc.subject.keywordsPoly (ethylene glycol)-grafted membrane-mimetic surfacesen_US
dc.subject.keywordsDPPE-Succinylen_US
dc.subject.keywordsDPPE-PEG2000en_US
dc.subject.keywordsMonolayers as model membranesen_US
dc.subject.keywordsNegatively Charged PEG-grafted Membranesen_US
dc.subject.keywordsSUVsen_US
dc.titleEffect of Saline and Non-Specific Insulin Binding on the Phase Behavior of Poly (Ethylene Glycol)-Grafted Phosphoethanolamine-Succinyl Model Membranesen_US
dc.typeElectronic Thesis or Dissertation

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