Cooperative binding by a bifunctional deoxycholic acid and cocaine-binding aptamer

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dc.contributor.authorDawood, Nusaibah E
dc.contributor.authorSlavkovic, Sladjana
dc.contributor.authorQureshi, Ruqaiya
dc.contributor.authorKhamissi, Natalie
dc.contributor.authorBauer, Christina
dc.contributor.authorReinstein, Oren
dc.contributor.authorJohnson, Philip E
dc.date.accessioned2022-07-05T11:12:53Z
dc.date.available2022-07-05T11:12:53Z
dc.date.issued2021-11-17
dc.description.abstractA bifunctional cocaine and deoxycholic acid-binding aptamer was constructed from the individual ligand binding aptamers and the binding affinity and thermodynamics were measured using isothermal titration calorimetry. We show that the bifunctional aptamer binds its ligands with positive cooperativity, having a Hill coefficient of 1.2-1.5, whether the ligands are added individually or as an equimolar mixture. When the helix between the binding sites is decreased from eight to six base pairs the Hill coefficient increased indicating a greater degree of cooperativity. Reducing the distance between the binding sites to four base pairs resulted in the bifunctional aptamer displaying negative cooperativity as the two sites are now too close to function efficiently. A mechanism where molecular motion, or dynamics, at one ligand-binding site are affected by the presence of the ligand at the other is proposed to account for the observed cooperative binding.en_US
dc.identifier.citationAptamers, 5, 2021, 31-38en_US
dc.identifier.issn2514-3247
dc.identifier.urihttp://hdl.handle.net/10315/39510
dc.language.isoenen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen_US
dc.rights.journalhttp://libpubmedia.co.uk/cooperative-binding-by-a-bifunctional-deoxycholic-acid-and-cocaine-binding-aptamer/en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleCooperative binding by a bifunctional deoxycholic acid and cocaine-binding aptameren_US
dc.typeArticleen_US

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