The Role Of Vab-3 in the Caenorhabditis Elegans Oxidative Stress Response

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dc.contributor.advisorKubiseski, Terrance J.
dc.contributor.authorYeo, Joon Yeob
dc.date.accessioned2021-03-08T17:29:10Z
dc.date.available2021-03-08T17:29:10Z
dc.date.copyright2020-12
dc.date.issued2021-03-08
dc.date.updated2021-03-08T17:29:09Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThe oxidative stress response protects the cell by balancing the number of free radicals inside the cell. BRAP2 is a Ras effector with ubiquitin ligase activity and conserved in C. elegans. Our lab has previously shown that C. elegans BRAP-2 is a negative regulator of SKN-1/Nrf2 that activates ROS detoxifying enzymes, and vab-3/PAX6 RNAi induction in brap-2 mutants reduced basal expression levels of gst-4. Based on the link between SKN-1 and BRAP-2, I examined the role of VAB-3 in the oxidative stress response. I found that a loss of vab-3 caused decreased basal expression levels of gst-4 in brap-2; vab-3 double mutants, decreased expression levels of gst-4, sdz-8, ugt-13 and ctl-1 and increased mortality rates toward oxidative stress in C. elegans. Moreover, I found that VAB-3 together with SKN-1 synergistically enhanced activities of gst-4 and skn-1c promoters. Thus, VAB-3 contributes to the oxidative stress response through the SKN-1/Nrf2 pathway.
dc.identifier.urihttp://hdl.handle.net/10315/38234
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectGenetics
dc.subject.keywordsbiology
dc.subject.keywordsMolecular biology
dc.subject.keywordsGenetics
dc.subject.keywordsOxidative
dc.subject.keywordsStress
dc.subject.keywordsTranscription
dc.subject.keywordsFactor
dc.subject.keywordsRegulation
dc.subject.keywordsOrtholog
dc.titleThe Role Of Vab-3 in the Caenorhabditis Elegans Oxidative Stress Response
dc.typeElectronic Thesis or Dissertation

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