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A non-canonical role for p107 in muscle stem cell fate decisions

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dc.contributor.advisorScime, Anthony
dc.contributor.authorHsiung, Justin Wei-Liang
dc.date.accessioned2024-03-18T17:59:45Z
dc.date.available2024-03-18T17:59:45Z
dc.date.issued2024-03-16
dc.date.updated2024-03-16T10:48:19Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThe regenerative potential of skeletal muscle is attributed to the presence of resident muscle stem cells known as satellite cells (SCs). After activation SCs have two fates, either to differentiate into new muscle or self-renew to replenish their population. Dysregulation in favor of one outcome over the other has been implicated in loss of muscular regenerative capacity. Mitochondrial metabolism has recently emerged as a regulator of SC fate decisions. We uncovered a non-canonical mitochondrial role for retinoblastoma-like protein 1 (Rbl1, p107) in manipulating fate decisions through its effect on mitochondrial dynamics. We find that in the absence of mitochondrially localized p107, SCs display a higher rate of self-renewal, while also exhibiting higher mitochondrial connectivity. This was associated with increases in the mitochondrial fusion protein OPA1, as well as a loss in cellular acetylation. Taken together, these findings suggest that non-canonical p107 function in SCs controls their cell fate decisions.
dc.identifier.urihttps://hdl.handle.net/10315/41884
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subjectMolecular biology
dc.subject.keywordsStem cells
dc.subject.keywordsStem cell
dc.subject.keywordsMuscle
dc.subject.keywordsMetabolism
dc.subject.keywordsMitochondria
dc.subject.keywordsp107
dc.subject.keywordsRbl1
dc.subject.keywordsRetinoblastoma-like protein
dc.subject.keywordsSelf-renewal
dc.subject.keywordsFate decision
dc.subject.keywordsMuscle stem cell
dc.subject.keywordsSatellite cell
dc.subject.keywordsGlycolysis
dc.subject.keywordsMitochondria fusion
dc.subject.keywordsMitochondria fission
dc.subject.keywordsMitochondrial dynamics
dc.subject.keywordsOPA1
dc.subject.keywordsOptic atrophy protein
dc.subject.keywordsNAD
dc.subject.keywordsNADH
dc.subject.keywordsNAD+
dc.titleA non-canonical role for p107 in muscle stem cell fate decisions
dc.typeElectronic Thesis or Dissertation

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