Effects of -Hydroxybutyrate (HB) on L6 Muscle cell Glucose and Fat Metabolism

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dc.contributor.advisorCeddia, Rolando
dc.creatorHadday, Ishvinder Singh
dc.date.accessioned2019-07-02T16:16:03Z
dc.date.available2019-07-02T16:16:03Z
dc.date.copyright2019-03-14
dc.date.issued2019-07-02
dc.date.updated2019-07-02T16:16:03Z
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThis study investigated the effects of the ketone body -hydroxybutyrate (HB) on glucose and fat metabolism in skeletal muscle cells. To accomplish this, L6 muscle cells were incubated with various HB concentrations (0.5, 1.0, 1.5, 20, and 2.5 mM) for 1, 3, and 5 days. Here, we report that HB significantly increased glucose uptake and its metabolism in L6 muscle cells. Time course analysis revealed that as early as 1 day of exposure to 1mM HB significantly enhanced basal and insulin-stimulated glucose uptake (1.6-fold), glycogen synthesis (1.3-fold), glucose oxidation (1.5-fold), and lactate production (1.3-fold) in skeletal muscle cells. Even though carnitine palmitoyl transferase 1 (CPT1) content and AMP-activated protein kinase (AMPK) phosphorylation significantly increased, no alteration was found in palmitate oxidation with HB treatment. Surprisingly, protein kinase B (AKT) phosphorylation was significantly reduced in muscle cells exposed to HB. In summary, HB increased AMPK phosphorylation and enhanced glucose uptake without altering fatty acid oxidation in skeletal muscle cells.
dc.identifier.urihttp://hdl.handle.net/10315/36281
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectKinesiology
dc.subject.keywordsβHB
dc.subject.keywordsBeta
dc.subject.keywordshydroxybutyrate
dc.subject.keywordsbeta hydroxybutyrate
dc.subject.keywordsketone
dc.subject.keywordsketogenic
dc.subject.keywordsketogenic diet
dc.subject.keywordsdiet
dc.subject.keywordsacetoacetate
dc.subject.keywordsaetone
dc.subject.keywordsAMPK
dc.subject.keywordsAKT
dc.subject.keywordsCPT1B
dc.subject.keywordsGSK3
dc.subject.keywordsGS
dc.subject.keywordscarnitine palmitoyl transferase 1
dc.subject.keywordsMP-activated protein kinase
dc.subject.keywordsprotein kinase B
dc.subject.keywordsglycogen synthase
dc.subject.keywordsglycogen synthase kinase
dc.subject.keywordslipids
dc.subject.keywordscarbohydrates
dc.subject.keywordsglucose
dc.subject.keywordsfat
dc.titleEffects of -Hydroxybutyrate (HB) on L6 Muscle cell Glucose and Fat Metabolism
dc.typeElectronic Thesis or Dissertation

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