Investigation of EPO-Mediated Rescue From P53-Dependent Apoptosis in DA3-EPOR Cells
dc.contributor.advisor | Benchimol, Samuel | |
dc.creator | Pham, Thuc-Nghi Duc | |
dc.date.accessioned | 2016-11-25T14:21:55Z | |
dc.date.available | 2016-11-25T14:21:55Z | |
dc.date.copyright | 2016-08-19 | |
dc.date.issued | 2016-11-25 | |
dc.date.updated | 2016-11-25T14:21:53Z | |
dc.degree.discipline | Biology | |
dc.degree.level | Master's | |
dc.degree.name | MSc - Master of Science | |
dc.description.abstract | The usage of recombinant human erythropoietin in clinics to treat cancer-associated anemia has shown unfortunate unforeseen tumour response to cytokine treatment. Although other cytokines have previously been shown to have an effect on p53-dependent tumourigenesis and apoptosis in vitro, the effects of erythropoietin on cancer development have only recently been observed in vivo, of which a potential mechanism has yet to be elucidated. To determine the potential mechanism by which erythropoietin mediates the evasion of apoptosis in cells, we used the wild-type p53-expressing murine leukemic cell line DA3-EPOR and induced apoptosis via daunorubicin and doxorubicin treatment. Our findings suggest that EPO rescues cells from p53-dependent apoptosis and enhances proteasomal degradation of p53 with a concomitant decrease in miR-34a, miR-34b/c, and lincRNA-p21 expression. EPO was also observed to increase p53 recruitment to the p21 promoter followed by increased p21 expression, suggesting an orchestrated shift from p53-dependent apoptosis to cell cycle arrest. | |
dc.identifier.uri | http://hdl.handle.net/10315/32801 | |
dc.language.iso | en | |
dc.rights | Author owns copyright, except where explicitly noted. Please contact the author directly with licensing requests. | |
dc.subject | Biology | |
dc.subject.keywords | p53 | |
dc.subject.keywords | Erythropoietin | |
dc.subject.keywords | Leukemia | |
dc.subject.keywords | Apoptosis | |
dc.subject.keywords | Cell cycle arrest | |
dc.subject.keywords | p53 target genes | |
dc.title | Investigation of EPO-Mediated Rescue From P53-Dependent Apoptosis in DA3-EPOR Cells | |
dc.type | Electronic Thesis or Dissertation |
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