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Investigation of EPO-Mediated Rescue From P53-Dependent Apoptosis in DA3-EPOR Cells

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dc.contributor.advisorBenchimol, Samuel
dc.creatorPham, Thuc-Nghi Duc
dc.date.accessioned2016-11-25T14:21:55Z
dc.date.available2016-11-25T14:21:55Z
dc.date.copyright2016-08-19
dc.date.issued2016-11-25
dc.date.updated2016-11-25T14:21:53Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThe usage of recombinant human erythropoietin in clinics to treat cancer-associated anemia has shown unfortunate unforeseen tumour response to cytokine treatment. Although other cytokines have previously been shown to have an effect on p53-dependent tumourigenesis and apoptosis in vitro, the effects of erythropoietin on cancer development have only recently been observed in vivo, of which a potential mechanism has yet to be elucidated. To determine the potential mechanism by which erythropoietin mediates the evasion of apoptosis in cells, we used the wild-type p53-expressing murine leukemic cell line DA3-EPOR and induced apoptosis via daunorubicin and doxorubicin treatment. Our findings suggest that EPO rescues cells from p53-dependent apoptosis and enhances proteasomal degradation of p53 with a concomitant decrease in miR-34a, miR-34b/c, and lincRNA-p21 expression. EPO was also observed to increase p53 recruitment to the p21 promoter followed by increased p21 expression, suggesting an orchestrated shift from p53-dependent apoptosis to cell cycle arrest.
dc.identifier.urihttp://hdl.handle.net/10315/32801
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subject.keywordsp53
dc.subject.keywordsErythropoietin
dc.subject.keywordsLeukemia
dc.subject.keywordsApoptosis
dc.subject.keywordsCell cycle arrest
dc.subject.keywordsp53 target genes
dc.titleInvestigation of EPO-Mediated Rescue From P53-Dependent Apoptosis in DA3-EPOR Cells
dc.typeElectronic Thesis or Dissertation

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