Development of a Workflow for a Robust Cytometry of Reaction Rate Constant

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dc.contributor.advisorKrylov, Sergey N.
dc.contributor.authorYosief, Robel
dc.date.accessioned2022-12-14T16:37:25Z
dc.date.available2022-12-14T16:37:25Z
dc.date.copyright2022-08-05
dc.date.issued2022-12-14
dc.date.updated2022-12-14T16:37:25Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractCytometry of Reaction Rate Constant (CRRC) is a method for studying cell population heterogeneity based on the activity of cellular processes. The original CRRC workflow did not account for cell motility, which led to inaccurate measurements in motile cells. Here, we report on the development of a new CRRC workflow that makes it applicable to motile cells. We confirmed the robustness of the new workflow to cell movement by performing a comparative CRRC workflow study of cross-membrane transport in motile cells. Using the new workflow, preliminary progress was made on the investigation of cytochrome p450 (CYP) activity. We validated the CRRC experimental procedure to conduct such study but found that the CRRC CYP assay had considerable variability. Nonetheless, the development of the new CRRC workflow is a step in the right direction with more work needing to be done to understand the variability found in the CRRC CYP assay.
dc.identifier.urihttp://hdl.handle.net/10315/40735
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subjectBiochemistry
dc.subjectAnalytical chemistry
dc.subject.keywordsBiomarkers
dc.subject.keywordsCytometry
dc.subject.keywordsChemoresistance
dc.subject.keywordsCell tracking
dc.subject.keywordsEnzyme kinetics
dc.titleDevelopment of a Workflow for a Robust Cytometry of Reaction Rate Constant
dc.typeElectronic Thesis or Dissertation

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