Investigating the Role of Selected Epigenetic Silencing Marks in Determining Mouse Skeletal Muscle Oxidative Phenotype

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dc.contributor.advisorRoudier, Emilie
dc.contributor.authorGulri, Manpreet Kaur
dc.date.accessioned2020-11-13T13:47:48Z
dc.date.available2020-11-13T13:47:48Z
dc.date.copyright2020-07
dc.date.issued2020-11-13
dc.date.updated2020-11-13T13:47:48Z
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractEpigenetic regulation of gene expression supports skeletal muscle phenotypic plasticity. The role of epigenetic silencing marks in maintaining muscle phenotype and in supporting muscle plasticity remains unknown. This study aims to characterize whether silencing marks established by Mdm2 and EZH2 play a role in determining the muscle oxidative phenotype at rest and after training. In sedentary C57Bl/6 female mice, the soleus muscle showed higher protein levels of Mdm2 and H3K27me3, and lower levels of EZH2 compared to glycolytic muscles. 9 weeks of endurance exercise training changed the protein levels of Mdm2, EZH2, and H3K27me3 in a muscle-specific manner in C57Bl/6 mice. Differentiation of C2C12 myoblasts depleted EZH2 protein levels, and increased the protein levels of Mdm2 and H3K27me3. Overall, our study shows that phenotypically different muscles show a different relative abundance of silencing marks. Endurance training may also affect the presence of silencing marks.
dc.identifier.urihttp://hdl.handle.net/10315/37891
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectKinesiology
dc.subject.keywordsEpigenetic
dc.subject.keywordsSilencing marks
dc.subject.keywordsHistone modification
dc.subject.keywordsGenes
dc.subject.keywordsGene silencing
dc.subject.keywordsGene repression
dc.subject.keywordsGene activation
dc.subject.keywordsTranscriptional repression
dc.subject.keywordsTranscriptional activation
dc.subject.keywordsh3k27me3
dc.subject.keywordsezh2
dc.subject.keywordsmdm2
dc.subject.keywordsExercise
dc.subject.keywordsEndurance
dc.subject.keywordsEndurance exercise
dc.subject.keywordsEndurance training
dc.subject.keywordsExercise training
dc.subject.keywordsSkeletal muscle
dc.subject.keywordsMouse
dc.subject.keywordsMice
dc.subject.keywordsc57bl6
dc.subject.keywordsc2c12
dc.subject.keywordsc2c12 differentiation
dc.subject.keywordsc2c12 myoblasts
dc.subject.keywordsLong term training
dc.subject.keywordsShort term training
dc.subject.keywordsMuscle phenotype
dc.subject.keywordsSkeletal muscle phenotype
dc.subject.keywordsOxidative muscle
dc.subject.keywordsGlycolytic muscle
dc.subject.keywordsSlow-twitch
dc.subject.keywordsFast-twitch
dc.subject.keywordsKinesiology
dc.subject.keywordsPhysiology
dc.subject.keywordsGenetics
dc.subject.keywordsPost-translational modifications
dc.subject.keywordsPtms
dc.subject.keywordsHistone methyltransferase
dc.subject.keywordse3 ubiquitin ligase
dc.subject.keywordsprc2
dc.subject.keywordsPolycomb recessive complex 2
dc.titleInvestigating the Role of Selected Epigenetic Silencing Marks in Determining Mouse Skeletal Muscle Oxidative Phenotype
dc.typeElectronic Thesis or Dissertation

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