Structural and Functional Studies of TraG From the F Plasmid and the Major Pilin From Coxiella Burnetii

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dc.contributor.advisorAudette, Gerald F.
dc.creatorErdogan, Fettah
dc.date.accessioned2017-07-26T15:31:46Z
dc.date.available2017-07-26T15:31:46Z
dc.date.copyright2016-08-15
dc.date.issued2017-07-26
dc.date.updated2017-07-26T15:31:46Z
dc.degree.disciplineChemistry
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThe Escherichia coli F-plasmid houses all genes necessary for the assembly of its conjugative machinery to facilitate successful DNA transfer. TraG is one of the largest proteins (102 kDa) encoded in the transfer (tra) region of the F-plasmid that facilitates conjugative transfer. The N-terminal region of TraG is involved in pilus synthesis and assembly, while the C-terminal periplasmic domain is required for plasmid-specific entry exclusion and mating pair stabilization. In order to provide structural insights into TraGs function, we have expressed, purified and initiated crystallization studies of the C-terminal region of TraG (designated TraG*, 52.7 kDa) and have obtained initial crystals of TraG*. Our experiments on functional and dynamic characterization of TraG* using liquid chromatography coupled mass spectrometry (LC-MS), size exclusion chromatography coupled multi-angle light scattering (SEC-MALS) and protein gel electrophoresis has given significant insight into TraG* crystallization strategies as well as potential TraG mechanism of action during conjugation.
dc.identifier.urihttp://hdl.handle.net/10315/33351
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectChemistry
dc.subject.keywordsX ray crystallography
dc.subject.keywordsTraG
dc.subject.keywordsF plasmid
dc.subject.keywordsTra region
dc.subject.keywordsTransfer protein
dc.subject.keywordsEscherichia coli
dc.subject.keywordsE coli
dc.subject.keywordsCoxiella burnettii
dc.subject.keywordsTraG*
dc.subject.keywordsTruncated ulp1
dc.subject.keywordsStructural biochemistry
dc.subject.keywordsBiological chemistry
dc.subject.keywordscbu0156
dc.subject.keywordspilA
dc.subject.keywordsT4SS
dc.subject.keywordsT2SS
dc.subject.keywordsSecretion systems
dc.subject.keywordsTransfer systems
dc.subject.keywordsBacterial conjugation
dc.subject.keywordsF conjugation
dc.subject.keywordsPilin
dc.subject.keywordsPili
dc.subject.keywordsMating pair stabilization
dc.subject.keywordsMating pair formation
dc.subject.keywordsEntry exclusion
dc.subject.keywordsPilin synthesis and assembly
dc.titleStructural and Functional Studies of TraG From the F Plasmid and the Major Pilin From Coxiella Burnetii
dc.typeElectronic Thesis or Dissertation

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