MicroRNA-378a-5p inhibits the differentiation of cytotrophoblast into syncytiotrophoblast

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dc.contributor.advisorPeng, Chun
dc.contributor.advisorSheng, Yi
dc.creatorNadeem, Uzma
dc.date.accessioned2016-09-13T13:14:15Z
dc.date.available2016-09-13T13:14:15Z
dc.date.copyright2013-08
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractMicroRNAs are expressed abundantly in the placenta throughout pregnancy. We have previously reported that miR-378a-5p promotes trophoblast cell proliferation and invasion. To further understand the role of miR-378a-5p during placental development, we determined if it may also regulate the differentiation of cytotrophoblast (CTB) into the syncytiotrophoblast (STB). Using BeWo cells, we found that miR-378a-5p was down-regulated during Forskolin-induced STB differentiation. Transfection of miR-378a-5p mimic into BeWo cells decreased the formation of multinucleated STB, mRNA levels of differentiation markers and E-cadherin levels. However, inhibition of endogenous miR-378a-5p by anti-miR-378a-5p increased cell fusion, induced STB marker gene expression and decreased E-cadherin levels. Luciferase reporter assay and real-time PCR analysis showed that CCNG2 is a target of miR-378a-5p. Taken together, these findings demonstrate that miR-378a-5p is a negative regulator of STB differentiation. This study also provides initial evidence that CCNG2 promotes trophoblast differentiation and may be involved in miR-378a-5p-regulated STB formation.
dc.identifier.urihttp://hdl.handle.net/10315/31952
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subject.keywordsMicroRNA-378a-5p
dc.subject.keywordsmiR-378a-5p
dc.subject.keywordscytotrophoblast
dc.subject.keywordsCTB
dc.subject.keywordssyncytiotrophoblast
dc.subject.keywordsSTB
dc.subject.keywordsCCNG2
dc.titleMicroRNA-378a-5p inhibits the differentiation of cytotrophoblast into syncytiotrophoblast
dc.typeElectronic Thesis or Dissertation

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