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Binding Determinants of HIV-1 REV RNA to the Pokeweed Antiviral Protein

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dc.contributor.advisorHudak, Katalin A.
dc.creatorJobst, Kass Alvin
dc.date.accessioned2015-08-28T15:35:58Z
dc.date.available2015-08-28T15:35:58Z
dc.date.copyright2015-04-21
dc.date.issued2015-08-28
dc.date.updated2015-08-28T15:35:58Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThe pokeweed antiviral protein (PAP) is an N-glycosidase that removes an adenine residue from the sarcin/ricin loop (SRL) of rRNA through a process called depurination. PAP has also been shown to depurinate the ORF of Rev RNA of HIV-1 in vivo without causing toxicity. The sequence and structure of Rev RNA that PAP interacts is identified and compared to the sarcin/ricin loop in order to describe the importance of RNA structure for PAP specificity. My results show that PAP binds to a short GGGAA sequence at the site of depurination of Rev RNA. Structural analysis reveals that the binding site is within a 15 nt hairpin pentaloop. The pentaloop contains a pseudo GNRA loop structure that swings G and A residues out of the hairpin, allowing PAP access to these purines for binding and depurination. These data provide new insight into the specificity of PAP to target an RNA.
dc.identifier.urihttp://hdl.handle.net/10315/30070
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiochemistry
dc.subjectMolecular biology
dc.subjectBiology
dc.subject.keywordsRNA
dc.subject.keywordsProtein RNA interaction
dc.subject.keywordsHIV-1
dc.subject.keywordsBiochemistry
dc.subject.keywordsPokeweed antiviral protein
dc.subject.keywordsPhytolacca americana
dc.subject.keywordsSarcin/Ricin loop
dc.subject.keywordsRNA structure.
dc.titleBinding Determinants of HIV-1 REV RNA to the Pokeweed Antiviral Protein
dc.typeElectronic Thesis or Dissertationen_US

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