Examining the Impact of PUFAs and the Role of Autophagy in Iron-Induced Ferroptotic Cell Death

Simple item page
dc.contributor.advisorSweeney, Gary
dc.contributor.authorDe Menezes Reboucas, Patricia
dc.date.accessioned2022-12-14T16:37:32Z
dc.date.available2022-12-14T16:37:32Z
dc.date.copyright2022-08-16
dc.date.issued2022-12-14
dc.date.updated2022-12-14T16:37:32Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractFerroptosis, an iron-dependent non-apoptotic cell death involves lipid peroxidation and associates with cardiometabolic disease and cancer. I established a model of iron overload in L6 cells and measured cell viability, toxicity and lipid peroxidation. Importantly, in PUFA pre-treated cells, the susceptibility to iron-induced lipid peroxidation was enhanced. This resulted in ferroptosis as cell death was attenuated by ferrostatin-1, a ferroptosis inhibitor, yet no attenuation was seen when an inhibitor of apoptosis was used. Increased lipid peroxidation was also found in PUFA pre-treaded cells in wild type but not in autophagy deficient cells. Additionally, preliminary data indicated an apparent rescue of ferroptosis-induced cell death when using the adiponectin receptor agonist ALY688, an autophagy inducer. In conclusion, these findings supported the theory that PUFAs can alter the cell susceptibility to iron-induced ferroptotic cell death and raise the possibility that autophagy plays a protective role against ferroptosis.
dc.identifier.urihttp://hdl.handle.net/10315/40736
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectMolecular biology
dc.subjectBiology
dc.subjectCellular biology
dc.subject.keywordsFerroptosis
dc.subject.keywordsIron overload
dc.subject.keywordsPUFAs
dc.subject.keywordsAutophagy
dc.subject.keywordsAdiponectin
dc.titleExamining the Impact of PUFAs and the Role of Autophagy in Iron-Induced Ferroptotic Cell Death
dc.typeElectronic Thesis or Dissertation

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
deMenezesReboucas_Patricia_2022_Masters.pdf
Size:
2.66 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
license.txt
Size:
1.87 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
YorkU_ETDlicense.txt
Size:
3.39 KB
Format:
Plain Text
Description:
Download

Collections

Biology

All items in the YorkSpace institutional repository are protected by copyright, with all rights reserved except where explicitly noted.