An adiponectin-S1P axis protects against lipid induced insulin resistance and cardiomyocyte cell death via reduction of oxidative stress

dc.contributor.authorBotta, Amy
dc.contributor.authorLiu, Ying
dc.contributor.authorWannaiampikul, Sivaporn
dc.contributor.authorTungtrongchitr, Rungsunn
dc.contributor.authorDadson, Keith
dc.contributor.authorPark, Tae-Sik
dc.contributor.authorSweeney, Gary
dc.date.accessioned2020-03-11T15:39:01Z
dc.date.available2020-03-11T15:39:01Z
dc.date.issued2019-02-21
dc.description.abstractBackground: Adiponectin exerts several beneficial cardiovascular effects, however their specific molecular mechanisms require additional understanding. This study investigated the mechanisms of adiponectin action in the heart during high fat diet (HFD) feeding or in palmitate (PA) treated H9c2 cardiomyoblasts. Methods: 6-week-old male adiponectin knock out (Ad-KO) mice were fed chow or 60% HFD for 6 weeks then received saline or recombinant adiponectin (3μg/g body weight) for an additional 2 weeks. After acute insulin stimulation (4 U/kg), tissue and serum samples were collected for analysis. H9c2 cardiomyocytes were treated ±0.1 mM PA, the adiponectin receptor agonist AdipoRon, or the antioxidant MnTBAP then assays to analyze reactive oxygen species (ROS) production and cell death were conducted. To specifically determine the mechanistic role of S1P, gain and loss of function studies were conducted with adding S1P to cells or the inhibitors THI and SKI-II, respectively. Results: HFD feeding induced cardiac insulin resistance in Ad-KO mice, which was reversed following replenishment of normal circulating adiponectin levels. In addition, myocardial total triglyceride was elevated by HFD and lipidomic analysis showed increased levels of ceramides and sphingosine-1 phosphate (S1P), with only the latter being corrected by adiponectin administration. Similarly, treatment of H9C2 cardiomyoblasts with PA led to a significant increase of intracellular S1P but not in conditioned media whereas AdipoRon significantly increased S1P production and secretion from cells. AdipoRon or the antioxidant MnTBAP significantly reduced PA-induced cell death. Gain and loss of function studies suggested S1P secretion and autocrine receptor activation mediated the effect of AdipoRon to attenuate PA-induced ROS production and cell death. Conclusion: Our data establish adiponectin signaling-mediated increase in S1P secretion as a mechanism via which HFD or PA induced cardiomyocyte lipotoxicity, leading to insulin resistance and cell death, is attenuated. Keywords: Adiponectin, Sphingosine-1-phosphate, Ceramide, Cardiomyocyte apoptosis, High fat diet, Palmitate, ROSen_US
dc.description.sponsorshipYork University Librariesen_US
dc.identifier.citationNutrition & Metabolism 16 (2019): 14.en_US
dc.identifier.urihttps://doi.org/10.1186/s12986-019-0342-yen_US
dc.identifier.urihttps://hdl.handle.net/10315/37092
dc.language.isoenen_US
dc.publisherBiomed Centralen_US
dc.rightsAttribution 2.5 Canada*
dc.rights.articlehttps://doi.org/10.1186/s12986-019-0342-yen_US
dc.rights.journalhttps://nutritionandmetabolism.biomedcentral.com/en_US
dc.rights.publisherhttps://www.biomedcentral.com/en_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/ca/*
dc.subjectAdiponectinen_US
dc.subjectSphingosine-1-phosphateen_US
dc.subjectCeramideen_US
dc.subjectCardiomyocyte apoptosisen_US
dc.subjectHigh fat dieten_US
dc.subjectPalmitateen_US
dc.subjectROSen_US
dc.titleAn adiponectin-S1P axis protects against lipid induced insulin resistance and cardiomyocyte cell death via reduction of oxidative stressen_US
dc.typeArticleen_US

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