The Effect of Ischemia and High-Fat Diet on Pericyte Fate

Simple item page
dc.contributor.advisorHaas, Tara
dc.contributor.authorNader, George
dc.date.accessioned2021-11-15T15:58:13Z
dc.date.available2021-11-15T15:58:13Z
dc.date.copyright2021-08
dc.date.issued2021-11-15
dc.date.updated2021-11-15T15:58:13Z
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThe purpose of my thesis was to assess the effects of high fat (HF) diet and ischemia on skeletal muscle pericytes. Pericytes are mural cells on the abluminal surface of capillaries that contribute to capillary stabilization and the regulation of angiogenesis, but their roles in the events that occur during muscle recovery from ischemia in patients with or without metabolic disorders remain to be established. I hypothesized that the number of capillary-associated pericytes and their phenotype will be altered by HF diet and ischemia. I used mice with fluorescently labelled pericytes (Ng2/DsRed) that were fed normal or HF diet and then underwent unilateral femoral artery ligation surgery to induce ischemia. My data showed that ischemia increased pericyte number through proliferation. Pericyte phenotype and morphology changed with ischemia, together with increased transcript levels of destabilizing factor Angiopoietin 2 mRNA. Overall, this study can be the first step in understanding the participation of pericytes in ischemic muscle recovery.
dc.identifier.urihttp://hdl.handle.net/10315/38815
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectPhysiology
dc.subject.keywordsPericyte
dc.subject.keywordsIschemia
dc.subject.keywordsHigh fat diet
dc.subject.keywordsMicrocirculation
dc.subject.keywordsCapillary
dc.subject.keywordsSkeletal muscle
dc.titleThe Effect of Ischemia and High-Fat Diet on Pericyte Fate
dc.typeElectronic Thesis or Dissertation

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Nader_George_2021_Masters.pdf
Size:
1.14 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
license.txt
Size:
1.87 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
YorkU_ETDlicense.txt
Size:
3.39 KB
Format:
Plain Text
Description:
Download

Collections

Kinesiology & Health Science

All items in the YorkSpace institutional repository are protected by copyright, with all rights reserved except where explicitly noted.