Low-Cost and High-Throughput Optofluidic Add-on Device for Light Sheet Imaging of Larval and Adult C. Elegans
| dc.contributor.advisor | Tabatabaei, Nima | |
| dc.contributor.advisor | Rezai, Pouya | |
| dc.contributor.author | Rahimpouresfahani, Faraz | |
| dc.date.accessioned | 2024-03-18T18:15:38Z | |
| dc.date.available | 2024-03-18T18:15:38Z | |
| dc.date.issued | 2024-03-16 | |
| dc.date.updated | 2024-03-16T10:43:30Z | |
| dc.degree.discipline | Mechanical Engineering | |
| dc.degree.level | Master's | |
| dc.degree.name | MASc - Master of Applied Science | |
| dc.description.abstract | In this research, we have made modifications to our low-cost light sheet platform, allowing us to capture high-content cross-sectional images of nematodes' nervous systems at earlier stages of development and with higher throughputs. Our platform was put to the test in imaging larval and adult pan neural worms, as well as in analyzing control and neurotoxin-exposed worms. Additionally, we utilized a new transgenic worm model for Parkinson's disease and were able to detect a fluorescence difference between larval and adult stages. The result is a vast dataset of high-content images that will be invaluable in future research. | |
| dc.identifier.uri | https://hdl.handle.net/10315/41958 | |
| dc.language | en | |
| dc.rights | Author owns copyright, except where explicitly noted. Please contact the author directly with licensing requests. | |
| dc.subject | Mechanical engineering | |
| dc.subject.keywords | Light sheet | |
| dc.subject.keywords | Microscopy | |
| dc.subject.keywords | Low-cost | |
| dc.subject.keywords | C. elegans | |
| dc.subject.keywords | Optofluidic | |
| dc.title | Low-Cost and High-Throughput Optofluidic Add-on Device for Light Sheet Imaging of Larval and Adult C. Elegans | |
| dc.type | Electronic Thesis or Dissertation |
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