Describing the Ionoregulatory Changes that Underlie Cold Acclimation in Drosophila Melanogaster

Simple item page
dc.contributor.advisorDonini, Andrew
dc.creatorYerushalmi, Gil
dc.date.accessioned2018-11-21T13:40:52Z
dc.date.available2018-11-21T13:40:52Z
dc.date.copyright2018-06-13
dc.date.issued2018-11-21
dc.date.updated2018-11-21T13:40:52Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractAt low temperatures Drosophila lose the ability to regulate ion and water balance across the gut leading to a lethal accumulation of K+ in the haemolymph (hyperkalemia). Cold-acclimation can mitigate these ion imbalances, but the physiological mechanisms that facilitate this process are still not understood. Upon adult emergence, D. melanogaster females were subjected to seven days at 25C (warm-acclimation) or 10C (cold-acclimation). Cold-acclimation reduced the critical thermal minimum (CTmin), sped up recovery from chill coma, improved survival following cold stress, and mitigated cold-induced hyperkalemia. In parallel, cold-acclimated flies experienced increased Malpighian tubule fluid and K+ secretion and reduced rectal K+ reabsorption. These changes were independent of Na+/K+- or V-type H+-ATPases. These results suggest that modification of Malpighian tubule and gut mitigate cold-induced hyperkalemia. Additionally, I present preliminary findings on the effects of acclimation, sex, and blood-feeding on the cold tolerance of the arboviral disease vector mosquito, A. aegypti.
dc.identifier.urihttp://hdl.handle.net/10315/35479
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectAnimal sciences
dc.subject.keywordsInsect
dc.subject.keywordsCold tolerance
dc.subject.keywordsDrosophila
dc.subject.keywordsMelanogaster
dc.subject.keywordsAcclimation
dc.subject.keywordsCold acclimation
dc.subject.keywordsIon balance
dc.subject.keywordsMalpighian tubules
dc.subject.keywordsRectum
dc.subject.keywordsAedes
dc.subject.keywordsAegypti
dc.titleDescribing the Ionoregulatory Changes that Underlie Cold Acclimation in Drosophila Melanogaster
dc.typeElectronic Thesis or Dissertation

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Yerushalmi_Gil_Y_2018_Masters.pdf
Size:
5.35 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
license.txt
Size:
1.83 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
YorkU_ETDlicense.txt
Size:
3.38 KB
Format:
Plain Text
Description:
Download

Collections

Biology

All items in the YorkSpace institutional repository are protected by copyright, with all rights reserved except where explicitly noted.