Measurements of forces produced by the mitotic spindle using optical tweezers

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dc.contributor.authorFerraro-Gideon, Jessica
dc.contributor.authorSheykhani, Rozhan
dc.contributor.authorZhu, Qingyuan
dc.contributor.authorDuquette, Michelle L.
dc.contributor.authorBerns, Michael
dc.contributor.authorForer, Arthur
dc.date.accessioned2025-02-04T22:22:08Z
dc.date.available2025-02-04T22:22:08Z
dc.date.issued2013-03-13
dc.description.abstractWe used a trapping laser to stop chromosome movements in Mesostoma and crane-fly spermatocytes and inward movements of spindle poles after laser cuts across Potorous tridactylus (rat kangaroo) kidney (PtK2) cell half-spindles. Mesostoma spermatocyte kinetochores execute oscillatory movements to and away from the spindle pole for 1–2 h, so we could trap kinetochores multiple times in the same spermatocyte. The trap was focused to a single point using a 63× oil immersion objective. Trap powers of 15–23 mW caused kinetochore oscillations to stop or decrease. Kinetochore oscillations resumed when the trap was released. In crane-fly spermatocytes trap powers of 56–85 mW stopped or slowed poleward chromosome movement. In PtK2 cells 8-mW trap power stopped the spindle pole from moving toward the equator. Forces in the traps were calculated using the equation F = Q′P/c, where P is the laser power and c is the speed of light. Use of appropriate Q′ coefficients gave the forces for stopping pole movements as 0.3–2.3 pN and for stopping chromosome movements in Mesostoma spermatocytes and crane-fly spermatocytes as 2–3 and 6–10 pN, respectively. These forces are close to theoretical calculations of forces causing chromosome movements but 100 times lower than the 700 pN measured previously in grasshopper spermatocytes.
dc.description.sponsorshipThis work was supported by grants from the Canadian Natural Sciences and Engineering Council to A.F., York University Fieldwork and Research Cost Funds to J.F., and the Beckman Laser Institute Foundation and a grant from the Air Force Office of Scientific Research FA9550-10-1-0538 to M.B.
dc.identifier.citationFerraro-Gideon, J., Sheykhani, R., Zhu, Q., Duquette, M. L., Berns, M. W., & Forer, A. (2013). Measurements of forces produced by the mitotic spindle using optical tweezers. Molecular biology of the cell, 24(9), 1375–1386. https://doi.org/10.1091/mbc.E12-12-0901
dc.identifier.issn1939-4586
dc.identifier.urihttps://doi.org/10.1091/mbc.E12-12-0901
dc.identifier.urihttps://hdl.handle.net/10315/42619
dc.language.isoen
dc.publisherScientific Society Publisher Alliance
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.titleMeasurements of forces produced by the mitotic spindle using optical tweezers
dc.typeArticle

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