Characterizing the Effects of Jasplakinolide on Mesostoma Ehrenbergii Primary Spermatocytes to Study the Role of Actin during Chromosome Movement

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dc.contributor.advisorForer, Arthur
dc.contributor.authorPatel, Divya-Shree
dc.date.accessioned2021-11-15T15:54:32Z
dc.date.available2021-11-15T15:54:32Z
dc.date.copyright2021-08
dc.date.issued2021-11-15
dc.date.updated2021-11-15T15:54:32Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractMy thesis investigated actins involvement in chromosome movement and function in the spindle. Generally, its thought that microtubules drive chromosome movement. However, when microtubules are depolymerized using nocodazole in Mesostoma ehrenbergii, chromosomes continue to move. To determine what proteins may contribute to chromosome movement, I targeted actin using an actin-stabilizing drug, jasplakinolide. I targeted actin because it has been found in the meiotic spindle of other cell types. Additionally, myosin has been implicated in Mesostoma chromosome movement, suggesting actin may be present since they work together in other systems. Jasplakinolide altered chromosome oscillation speeds and caused the precocious furrow to move and ingress towards a single pole at random, causing one side of the cell to become larger. The spindle pole of the larger half-cell moved along the cell periphery towards the opposite pole. My results suggest actin is involved in chromosome movement along with other non-microtubule spindle elements.
dc.identifier.urihttp://hdl.handle.net/10315/38784
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectMolecular biology
dc.subject.keywordsChromosome movement
dc.subject.keywordsChromosome segregation
dc.subject.keywordsMeiosis
dc.subject.keywordsPrimary spermatocytes
dc.subject.keywordsFurrow
dc.subject.keywordsCell division
dc.subject.keywordsCleavage furrow
dc.subject.keywordsSpindle
dc.subject.keywordsMicrotubules
dc.subject.keywordsActin
dc.subject.keywordsMyosin
dc.subject.keywordsSpindle matrix
dc.subject.keywordsJasplakinolide
dc.subject.keywordsPrecocious furrow
dc.titleCharacterizing the Effects of Jasplakinolide on Mesostoma Ehrenbergii Primary Spermatocytes to Study the Role of Actin during Chromosome Movement
dc.typeElectronic Thesis or Dissertation

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