Investigating MDM2 Regulation of Endothelial Cell Angiomir Biosynthesis in Response to High Glucose Treatment

Simple item page
dc.contributor.advisorBirot, Olivier
dc.contributor.authorLam, Brian
dc.date.accessioned2020-05-11T12:41:54Z
dc.date.available2020-05-11T12:41:54Z
dc.date.copyright2019-08
dc.date.issued2020-05-11
dc.date.updated2020-05-11T12:41:53Z
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractMiRNAs are a class of short, non-coding RNAs that regulate gene expression at the post-transcriptional level. Dysregulation of miRNA production contributes to the pathogenesis of hyperglycemia-related complications. Although many studies have examined the effect of hyperglycemia on the expression of miRNAs, the effect of hyperglycemia specifically on the endothelial cell miRNA biogenesis machinery is poorly understood. The aim of my project is to better characterize the effect of high glucose treatment on the miRNA biogenesis machinery and miRNA expression in endothelial cells. HDMECs were incubated with low (5mM) and high (30mM) glucose concentrations for 6 and 24 hours and subsequent miRNA expression was measured. My results indicate that exposure to high glucose concentrations enhances MDM2:DROSHA binding and represses DROSHA expression both in vitro and in diabetic mice (db/db) muscles. Treatment with MDM2 inhibitors repealed glucose-induced DROSHA downregulation. This suggests that high glucose concentrations downregulates DROSHA expression through an MDM2-mediated mechanism.
dc.identifier.urihttps://hdl.handle.net/10315/37382
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectCellular biology
dc.subject.keywordsmiRNA
dc.subject.keywordsHyperglycemia
dc.subject.keywordsmdm2
dc.subject.keywordsDrosha
dc.subject.keywordsGlucose
dc.subject.keywordsEndothelial
dc.subject.keywordsCell
dc.titleInvestigating MDM2 Regulation of Endothelial Cell Angiomir Biosynthesis in Response to High Glucose Treatment
dc.typeElectronic Thesis or Dissertation

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Lam_Brian_2019_Masters.pdf
Size:
2.02 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
license.txt
Size:
1.83 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
YorkU_ETDlicense.txt
Size:
3.36 KB
Format:
Plain Text
Description:
Download

Collections

Kinesiology & Health Science

All items in the YorkSpace institutional repository are protected by copyright, with all rights reserved except where explicitly noted.