Characterization of PRD-1 Mutation in Neurospora crassa

Simple item page
dc.contributor.advisorLakin-Thomas, Patricia
dc.creatorFiroozi, Ghazaleh
dc.date.accessioned2014-07-14T20:57:32Z
dc.date.available2014-07-14T20:57:32Z
dc.date.copyright2013-12-18
dc.date.issued2014-07-09
dc.date.updated2014-07-09T16:30:01Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractIn Neurospora crassa, rhythmic conidiation is controlled by several oscillators such as FRQ/WCC and FLO. The frequency (frq) gene and white collar genes (WC-1 and WC-2) are the most important components of the FRQ/WCC oscillator and prd-1 and prd-2 genes could be important components of the FLO. This project aims to characterize the prd-1 mutation. This involved mapping the mutation using PCR analyses based on single nucleotide polymorphism (SNP) markers. After determining a minimal interval, candidate genes were sequenced, and knockout mutants were screened in this interval for prd-1 phenotype. A candidate gene was found to have a mutation that affects the splicing of the mRNA. The identity of the gene was confirmed by complementing the prd-1 mutant with a wild type copy of the identified candidate gene. The prd-1 gene is identified as an RNA helicase.en_US
dc.identifier.urihttp://hdl.handle.net/10315/27621
dc.language.isoenen_US
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiologyen_US
dc.subject.keywordsPrd-1 geneen_US
dc.subject.keywordsCircadian rhythmen_US
dc.subject.keywordsNeurosporaen_US
dc.titleCharacterization of PRD-1 Mutation in Neurospora crassaen_US
dc.typeElectronic Thesis or Dissertation

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Firoozi_Ghazaleh_2013_Masters.pdf
Size:
3.5 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
license.txt
Size:
1.83 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
No Thumbnail Available
Name:
YorkU_ETDlicense.txt
Size:
3.38 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Biology

All items in the YorkSpace institutional repository are protected by copyright, with all rights reserved except where explicitly noted.