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Pinpointing The Structural Dynamics of Plasminogen Activator Inhibitor 1 binding to Heparin using Hydrogen/Deuterium Exchange Mass Spectrometry

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dc.contributor.authorAbdalla, Marwa
dc.contributor.authorAnacleto, Joseph
dc.contributor.authorWilson, Derek
dc.date.accessioned2023-06-01T23:15:54Z
dc.date.available2023-06-01T23:15:54Z
dc.date.issued2023-05-17
dc.description.abstractPAI-1 is a key player to regulate the activation of fibrinolysis, with broad influence effects on inflammation, hemostasis, tissue remodeling, and wound healing 1 The binding of endogenous cofactor vitronectin to PAI 1 helps to extend PAI 1 half life and delay its latency transition thus controlling the stability of the active form 1 Previous studies have suggested that low molecular weight heparin alters the levels of circulating PAI 1 and enhances endogenous fibrinolysis However, the intrinsic dynamics of this binding are not completely understood 2 Our findings reveal that Low Molecular Weight Heparin n ( may contribute to the localization of PAI 1 at specific sites, hence involved in the regulation of plasminogen activation and its functional stability Hydrogen-Deuterium Exchange ( coupled to MS is widely used to study protein dynamics Continuous time resolved Electrospray ionization TRESI HDX MS technique is used to characterize protein structural transitions in relatively ordered regions of proteins 3en_US
dc.description.sponsorshipFunded by: funder-id http://dx.doi.org/10.13039/501100000038, Natural Sciences and Engineering Research Council of Canada; Award ID: RGPIN 480432 Funded by: funder-id http://dx.doi.org/10.13039/501100004489, Mitacsen_US
dc.identifier.citationMarwa ElSabaawy, Joseph Anacleto and Derek Wilson. Pinpointing The Structural Dynamics of Plasminogen Activator Inhibitor -1 upon binding to Heparin using Hydrogen/Deuterium Exchange Mass Spectrometry. ScienceOpen Posters. 2023. DOI: 10.14293/P2199-8442.1.SOP-.PMWQOQ.v1en_US
dc.identifier.urihttps://doi.org/10.14293/p2199-8442.1.sop-.pmwqoq.v1en_US
dc.identifier.urihttp://hdl.handle.net/10315/41189
dc.language.isoenen_US
dc.publisherScienceOpen Postersen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titlePinpointing The Structural Dynamics of Plasminogen Activator Inhibitor 1 binding to Heparin using Hydrogen/Deuterium Exchange Mass Spectrometryen_US
dc.typePosteren_US

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