Drosophila Melanogaster Oviposition and Toxicity Studies on a Chip

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dc.contributor.advisorRezai, Pouya
dc.creatorLeung, Jacob Ching Kan
dc.date.accessioned2016-09-20T16:41:10Z
dc.date.available2016-09-20T16:41:10Z
dc.date.copyright2015-12-10
dc.date.issued2016-09-20
dc.date.updated2016-09-20T16:41:10Z
dc.degree.disciplineEarth & Space Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractOviposition or egg-laying is an important behaviour used to assess the biological processes in Drosophila melanogaster. This behaviour is affected by physical and chemical properties of the substrate, which have not been investigated precisely and parametrically with existing manual approaches. Current oviposition-based chemical screening studies using agar plates are inaccurate, labor-intensive, and inflexible due to the manual chemical doping of agar. In this thesis, we have devised a miniaturization method to precisely and repeatedly manipulate agar stiffness and exposure area to quantitatively study their effects on oviposition in Drosophila. Using this method, we have also developed agar-polydimethylsiloxane microfluidic devices for single- and multi-concentration chemical (zinc and acetic acid) dosing and on-chip oviposition screening of Drosophila. A 1% exposure area was found to provide pure agar-like oviposition, and our microfluidic devices demonstrated chemical concentration dependency in oviposition responses. These devices may be further used for assaying fundamental oviposition questions, learning, and decision-making phenomena in Drosophila and other egg-laying insects.
dc.identifier.urihttp://hdl.handle.net/10315/32189
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subject.keywordsMicrofluidics
dc.subject.keywordsDrosophila melanogaster
dc.subject.keywordsFruit fly
dc.subject.keywordsAdult-staged
dc.subject.keywordsOviposition
dc.subject.keywordsViability
dc.subject.keywordsChemical screening
dc.subject.keywordsChemical dosing
dc.subject.keywordsAgar-Polydimethylsiloxane
dc.subject.keywordsHybrid devices
dc.subject.keywordsPolymer
dc.subject.keywordsFluidic devices
dc.subject.keywordsSurface patterning
dc.subject.keywordsChemical composition
dc.subject.keywordsOviposition Preference
dc.subject.keywordsToxicology
dc.titleDrosophila Melanogaster Oviposition and Toxicity Studies on a Chip
dc.typeElectronic Thesis or Dissertation

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