The Sumoylation of General Transcription Factors Regulates Transcription in Saccharomyces cerevisiae

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dc.contributor.advisorRosonina, Emanuel
dc.contributor.authorBaig, Mohammad Sohaib
dc.date.accessioned2022-08-08T15:43:31Z
dc.date.available2022-08-08T15:43:31Z
dc.date.copyright2021-12-17
dc.date.issued2022-08-08
dc.date.updated2022-08-08T15:43:30Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractSumoylation is a post-translational modification which primarily targets nuclear substrates and can result in altered target protein activity, stability, conformation, and/or localization. Out of the six general transcription factors (GTFs), we have determined that transcription factor IID (TFIID) and transcription factor IIF (TFIIF) are targets of sumoylation. The functional impacts of GTF sumoylation were assayed using strains with elevated or reduced TFIID or TFIIF sumoylation. Using chromatin immunoprecipitation (ChIP), we found that GTF sumoylation does not substantially impact GTF-chromatin interactions but can impact the promoter-wide sumoylation level. Notably, TFIID and TFIIF sumoylation enhances RNAPII recruitment at various promoters likely via altered protein-protein interactions but does so through seemingly distinctive mechanisms. A ChIP-seq and an RNA-seq revealed that reduced GTF sumoylation results in diminished RNAPII density at thousands of genes but this change does not substantially impact steady-state mRNA levels. This research has uncovered novel characteristics of transcriptional regulation in eukaryotes.
dc.identifier.urihttp://hdl.handle.net/10315/39568
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subject.keywordsSumoylation
dc.subject.keywordsSUMO
dc.subject.keywordsRegulation
dc.subject.keywordsTranscription
dc.subject.keywordsTranscription factors
dc.subject.keywordsTFs
dc.subject.keywordsGeneral transcription factors
dc.subject.keywordsGTFs
dc.subject.keywordsTFIID
dc.subject.keywordsTATA-binding protein
dc.subject.keywordsTBP
dc.subject.keywordsTFIIF
dc.subject.keywordsTfg1
dc.subject.keywordsPost-translational modification
dc.subject.keywordsPTM
dc.subject.keywordsRNA polymerase II
dc.subject.keywordsRNAPII
dc.subject.keywordsRecruitment
dc.subject.keywordsChIP
dc.subject.keywordsChIP-qPCR
dc.subject.keywordsChIP-seq
dc.subject.keywordsRT-qPCR
dc.subject.keywordsRNA-seq
dc.subject.keywordsChromatin fractionation
dc.subject.keywordsYeast
dc.titleThe Sumoylation of General Transcription Factors Regulates Transcription in Saccharomyces cerevisiae
dc.typeElectronic Thesis or Dissertation

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