Now showing items 1-5 of 5
Measuring the Dynamics and Kinetics of OXA-58, a Class D Abeta-Lactamase from Acinetobacter Baumannii
OXA-58 is a β-lactamase enzyme that bacteria Acinetobacter baumannii employ as their chief defense mechanism against β-lactam antibiotic treatment. The catalytic activity of OXA-58 and its mutants was monitored as they ...
Mass Spectrometry Methods for Enhanced Characterization of Biological Systems
As the pharmaceutical industry moves towards larger protein-based therapeutics over chemically synthesized small molecules, there are increasing demands for parallel advancement in the technologies used for quality control ...
Characterizing the Structure-Function Relationship of Pathological Proteins
Protein structure and dynamics dictate the fundamentals of protein function. For example, a structured protein must change its conformation when interacting with various ligands. In addition, misfolded or intrinsically ...
Front-End Methods For Enhancing the Analytical Power of Mass Spectrometry
The analytical power and versatility of mass spectrometry can be enhanced by adding front-end devices, which provide additional functionality before, during or immediately after ElectroSpray Ionization (ESI). Such devices ...
Characterizing Conformational Dynamics and Catalytic Activity of Enzymes by Hydrogen-Deuterium Exchange Mass Spectrometry
Determining the conformational dynamics of enzymes as they undergo catalysis has been challenging for decades due to the short timescale and limitations of traditional analysis, including techniques such as CPMG 2D NMR and ...