The Role of Syntaxin-1A on Excitation-Contraction Coupling in the Adult Male and Female Mouse Heart

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dc.contributor.advisorTsushima, Robert G.
dc.contributor.authorVirdi, Amanvir
dc.date.accessioned2022-08-08T15:52:47Z
dc.date.available2022-08-08T15:52:47Z
dc.date.copyright2022-04-05
dc.date.issued2022-08-08
dc.date.updated2022-08-08T15:52:47Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractThe SNARE protein, syntaxin-1A (STX1A) is expressed in the adult rodent heart, yet its role is not fully established. This thesis explored STX1As role in cardiac excitation-contraction (EC) coupling in male and female mouse hearts. Heart-specific homozygous and heterozygous STX1A knockouts were achieved via a tamoxifen-inducible Cre-Lox system. Echocardiography revealed significant systolic dysfunction post-STX1A knockout (week 0), with recovery beginning at week 1 and an absence of sex differences in cardiac function. Transient thickening of the posterior wall (week 1) and delayed contractions (week 0-3) were seen. No genetic compensation of STX2, 3, or 4 was seen, however, EC coupling genes, Cav1.2, RyR2, and SERCA2a, declined at week 0 and recovered at week 3, aligning with the phenotype recovery. Control Cre+ mice remained unchanged, suggesting the transient systolic dysfunction stems from compromised transcription of Ca2+ handling genes. Therefore, STX1A may facilitate normal EC coupling gene expression in adult mouse hearts.
dc.identifier.urihttp://hdl.handle.net/10315/39629
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectCellular biology
dc.subject.keywordsSNARE
dc.subject.keywordsSyntaxin-1A
dc.subject.keywordsExcitation-contraction coupling
dc.subject.keywordsMammalian heart
dc.subject.keywordsLeft ventricle
dc.subject.keywordsHeart-specific STX1A KO
dc.subject.keywordsTransient systolic dysfunction
dc.subject.keywordsCalcium handling gene expression
dc.subject.keywordsEchocardiography
dc.subject.keywordsRT-qPCR
dc.subject.keywordsCardiac function
dc.subject.keywordsMouse heart
dc.subject.keywordsCardiac sex differences
dc.subject.keywordsTamoxifen-inducible Cre-Lox system
dc.subject.keywordsContractile function of heart
dc.subject.keywordsEndocrine function of heart
dc.titleThe Role of Syntaxin-1A on Excitation-Contraction Coupling in the Adult Male and Female Mouse Heart
dc.typeElectronic Thesis or Dissertation

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