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Genetic analysis of offspring of a female-female pair in the lesser snow goose (Chen caerulescens caerulescens).

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Date

1989

Authors

Quinn, T.W.
Davies, J.C.
Cooke, F.
White, B.N.

Journal Title

Journal ISSN

Volume Title

Publisher

University of California Press

Abstract

We used restriction-fragment-length polymorphism (RFLP) analysis to establish the parentage of a clutch of eight eggs being incubated by two female Lesser Snow Geese to determine if both females had contributed to the clutch, and whether a single male had fertilized both females. Genotypes at 30 polymorphic restriction enzyme sites were surveyed with 14 cloned DNA probes. Sexing of all individuals was done both by dissection and by use of a DNA probe that detected the presence of the W chromosome in females. Paternal genotypes were reconstructed from haplotypes of the offspring. We determined that both females had contributed to the clutch, and that each was fertilized by a different male. Received 9 May 1988, accepted 30 September 1988

Description

Keywords

OFFSPRING OF A FEMALE-FEMALE PAIR, LESSER SNOW GOOSE, CHEN C. CAERULESCENS

Citation

The Auk 106:177-184.1989