The Unfolded Protein Response in Relation to Mitochondrial Biogenesis in Skeletal Muscle Cells

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dc.contributor.advisorHood, David A.
dc.creatorMoosavi, Zahra Sadat Mesbah
dc.date.accessioned2017-07-27T12:35:43Z
dc.date.available2017-07-27T12:35:43Z
dc.date.copyright2016-10-25
dc.date.issued2017-07-27
dc.date.updated2017-07-27T12:35:43Z
dc.degree.disciplineKinesiology & Health Science
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractMitochondrial biogenesis involves nuclear- and mitochondrial-derived proteins to be integrated into functional organelles. Muscle development and chronic exercise are two physiological stimuli that trigger the production of mitochondrial components to produce more mitochondria. However, the synthesis of new proteins can induce cellular stress. Thus, the unfolded protein response (UPR), which takes place in the mitochondria or the endoplasmic reticulum, ensures correct protein handling. Whether the UPR must precede mitochondrial biogenesis is unknown. We used two models of mitochondrial biogenesis, skeletal muscle differentiation and chronic exercise of muscle cells in culture, and examined UPR activation. We partially inhibited one branch of the UPR involving the protein CHOP, with either a drug (TUDCA) or gene knockdown. Our results indicate that mitochondrial biogenesis occurs independently of stress-induced CHOP, and reducing ER stress may further augment mitochondrial content.
dc.identifier.urihttp://hdl.handle.net/10315/33429
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectPhysiology
dc.subject.keywordsUPR
dc.subject.keywordsUnfolded protein response
dc.subject.keywordsER
dc.subject.keywordsMitochondria
dc.subject.keywordsEndoplasmic reticulum
dc.subject.keywordsCCA
dc.subject.keywordsChronic contractile activity
dc.subject.keywordsMuscle cells
dc.subject.keywordsMyogenesis
dc.subject.keywordsMuscle development
dc.subject.keywordsDifferentiation
dc.subject.keywordsStress
dc.subject.keywordsBiogenesis
dc.subject.keywordsMitochondrial biogenesis
dc.subject.keywordsExercise
dc.subject.keywordsCHOP
dc.subject.keywordsCHOP-10
dc.subject.keywordsGADD153
dc.subject.keywordsATF4
dc.subject.keywordsATF6
dc.subject.keywordsMammalian cells
dc.subject.keywordsCell physiology
dc.subject.keywordsHood
dc.subject.keywordsC2C12
dc.subject.keywordsZahra
dc.subject.keywordsDavid hood
dc.subject.keywordsMurine cells
dc.titleThe Unfolded Protein Response in Relation to Mitochondrial Biogenesis in Skeletal Muscle Cells
dc.typeElectronic Thesis or Dissertation

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