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Identification of Regions Required for CDCA7 Interaction with DNA Damage Repair Machinery

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dc.contributor.advisorScheid, Michael
dc.contributor.authorJaff, Shaina Rachel
dc.date.accessioned2023-12-08T14:37:35Z
dc.date.available2023-12-08T14:37:35Z
dc.date.issued2023-12-08
dc.date.updated2023-12-08T14:37:35Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractCDCA7 (Cell Division Cycle Associated Protein 7) is a transcription factor protein that binds to DNA and histone modifying enzymes supporting DNA methylation and contributes to repair of double stranded breaks in DNA. Mutations of the cdca7 gene cause ICF (Immunodeficiency, centromeric instability, and facial abnormalities) syndrome. CDCA7 has been shown to bind with HELLS (Helicase, lymphoid specific) as a bipartite nucleosome remodeller to allow for de novo methylation by DNMT3b (DNA methyl transferase 3b). Additionally, CDCA7 associates with Ku70 and Ku80, proteins essential for DNA damage repair via the Non-Homologous End Joining (NHEJ) pathway, and -H2AX, whose accumulation is facilitated by Ku proteins and is a biomarker of DNA damage. I show here that CDCA7 requires a putative leucine zipper for association with HELLS, while the binding of 14-3-3 at a phosphorylated residue in CDCA7 regulates Ku70/80 and -H2AX association. This study further elucidates the mechanism of how CDCA7 plays a crucial role in maintaining genomic stability by participating in various DNA repair processes and DNA methylation.
dc.identifier.urihttps://hdl.handle.net/10315/41704
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subjectBiochemistry
dc.subjectCellular biology
dc.subject.keywordsMolecular biology
dc.subject.keywordsSignal transduction pathways
dc.subject.keywordsICF syndrome
dc.subject.keywordsProtein-protein interactions
dc.subject.keywordsTranscription factors
dc.subject.keywordsPI3K/AKT pathway
dc.subject.keywordsNon homologous end joining
dc.subject.keywordsDNA damage
dc.subject.keywordsDNA repair
dc.subject.keywordsHELLS
dc.subject.keywordsCDCA7
dc.subject.keywords14-3-3
dc.subject.keywordsyH2AX
dc.subject.keywordsKu80
dc.subject.keywordsKu
dc.subject.keywordsDNA methylation
dc.subject.keywordsDNMT3B
dc.subject.keywordsLeucine zipper
dc.titleIdentification of Regions Required for CDCA7 Interaction with DNA Damage Repair Machinery
dc.typeElectronic Thesis or Dissertation

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