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Hybrid Microfluidic Devices For On-Demand Manipulation and Screening of Neurons and Organs of Small Model Organisms

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dc.contributor.advisorRezai, Pouya
dc.creatorArdeshiri, Ramtin
dc.date.accessioned2017-07-26T15:28:52Z
dc.date.available2017-07-26T15:28:52Z
dc.date.copyright2016-08-15
dc.date.issued2017-07-26
dc.date.updated2017-07-26T15:28:52Z
dc.degree.disciplineMechanical Engineering
dc.degree.levelMaster's
dc.degree.nameMASc - Master of Applied Science
dc.description.abstractCaenorhabditis elegans and Drosophila melanogaster are widely used model organisms for neurological and cardiac studies due to their simple neuronal and cardiac systems, genome similarity to humans, and ease of maintenance in laboratories. However, their 50m-1mm sizes and continuous mobility impede their precise spatiotemporal manipulation, thereby, reducing the throughput of biological assays. By integrating glass capillaries into microfluidic devices and using 3D-printed fixtures for precise control, we have developed hybrid lab-on-a-chip devices to facilitate the processes of animal manipulation and stimuli control, using modules for single-organism selection, orientation, imaging and chemical stimulation. These microdevices enabled us to manipulate organisms individually and to orient them at any desired direction for imaging purposes. The applications of these hybrid microdevices were demonstrated in the optical and fluorescent imaging of C. elegans cells as well as cardiac screening of Drosophila larvae. This technique can be applied in fundamental biology, toxicology, and drug discovery.
dc.identifier.urihttp://hdl.handle.net/10315/33335
dc.language.isoen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectEngineering
dc.subject.keywordsMicrodevices
dc.subject.keywordsLab-on-a-chip
dc.subject.keywordsC. elegans
dc.subject.keywordsD. melanogaster
dc.subject.keywordsModel organism orientation
dc.subject.keywordsChemical screening
dc.subject.keywordsMicrofluidics
dc.titleHybrid Microfluidic Devices For On-Demand Manipulation and Screening of Neurons and Organs of Small Model Organisms
dc.typeElectronic Thesis or Dissertation

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