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Identifying Novel Interaction Between CDK8 Kinase Module and The Deubiquitinating Enzyme Ubiquitin Specific Protease 7 (USP7)

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dc.contributor.advisorSaridakis, Vivian
dc.contributor.authorBanyameen, Krstina Akram
dc.date.accessioned2023-12-08T14:21:18Z
dc.date.available2023-12-08T14:21:18Z
dc.date.issued2023-12-08
dc.date.updated2023-12-08T14:21:17Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractUbiquitin Specific Protease 7 (USP7) is a deubiquitinating enzyme that regulates and interacts with various cellular substrates, playing regulatory roles in various pathways such as DNA damage repair and epigenetics. This thesis explores the role of USP7 in regulating components of gene expression regulators. The gene expression of target genes is fine-tuned by the transcriptional co-activator, the Mediator complex. Our group has determined that Cyclin Dependent Kinase 8 (CDK8) and Mediator Complex Subunit 12 (MED12), components of the kinase module in the Mediator complex, contain consensus sequences for binding to the N-terminus of USP7. Using various co-immunoprecipitation, pulldowns, tissue culture, and western blotting related techniques, our findings show that USP7, using the DWGF motif at its N-terminus, interacts with and stabilizes protein levels of MED12 and CDK8. This provides insights onto the cellular role of USP7 in gene expression, giving such novel interactions therapeutic potential for diseases such as cancer.
dc.identifier.urihttps://hdl.handle.net/10315/41591
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectBiology
dc.subject.keywordsMolecular biology
dc.subject.keywordsGene expression
dc.subject.keywordsUbiquitination
dc.subject.keywordsDeubiquitylation
dc.subject.keywordsProtein-protein interaction
dc.subject.keywordsTissue culture
dc.subject.keywordsCo-immunoprecipitation
dc.subject.keywordsCancer
dc.titleIdentifying Novel Interaction Between CDK8 Kinase Module and The Deubiquitinating Enzyme Ubiquitin Specific Protease 7 (USP7)
dc.typeElectronic Thesis or Dissertation

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