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Determining the Genes for Distyly in Turnera

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dc.contributor.advisorShore, Joel S
dc.contributor.authorHamam, Hasan Jihad
dc.date.accessioned2019-11-22T18:49:57Z
dc.date.available2019-11-22T18:49:57Z
dc.date.copyright2019-07
dc.date.issued2019-11-22
dc.date.updated2019-11-22T18:49:57Z
dc.degree.disciplineBiology
dc.degree.levelMaster's
dc.degree.nameMSc - Master of Science
dc.description.abstractHeterostyly promotes outbreeding and reduces pollen wastage. Distylous species have two mating types: L-morph and S-morph plants, which are genetically determined by the S-locus. It was hypothesized that the S-locus carries at least three tightly linked genes constituting a supergene. The S-locus genes responsible for distyly in Turnera had not been determined. Using deletion mapping and chromosome walking, the S- and s-haplotypes of the S-locus were constructed. Three hemizygous genes, TsSPH1, TsYUC6, and TsBAHD occur only on the S-haplotype and appear to determine S-morph characteristics. TsSPH1 is expressed in filaments, TsYUC6 in anthers, and TsBAHD in styles. A long-homostyle mutant (Drh) did not possess TsBAHD while another long-homostyle mutant (Mhomo-H) possessed a substitution mutation in TsBAHD. Short-homostyle mutants did not express TsSPH1. The incompatibility system of Turnera joelii possessed a mutation that appears to be linked to S-locus. A possible mechanism of incompatibility is discussed which involves brassinosteroids and auxins.
dc.identifier.urihttp://hdl.handle.net/10315/36742
dc.languageen
dc.rightsAuthor owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.
dc.subjectMolecular biology
dc.subject.keywordsEvolution
dc.subject.keywordsTurnera
dc.subject.keywordsdistyly
dc.subject.keywordsgenetic mapping
dc.subject.keywordsheterostyly
dc.subject.keywordsS-locus
dc.titleDetermining the Genes for Distyly in Turnera
dc.typeElectronic Thesis or Dissertation

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