Now showing items 1-5 of 5
Measuring the Dynamics and Kinetics of OXA-58, a Class D Abeta-Lactamase from Acinetobacter Baumannii
OXA-58 is a β-lactamase enzyme that bacteria Acinetobacter baumannii employ as their chief defense mechanism against β-lactam antibiotic treatment. The catalytic activity of OXA-58 and its mutants was monitored as they ...
A Stable Dicationic Salt in Reactive DESI-MS Imaging in Positive Ion Mode to Analyze Biological Samples
A dicationic salt was applied for imaging in reactive desorption electrospray ionization mass spectrometry (DESI-MS) on biological samples. The samples interrogated in this experiment were whole body zebrafish tissues and ...
Obtaining Structural Insights on Bacterial Protein Complexes Using Time-Resolved Hydrogen-Deuterium Exchange Mass Spectrometry
Persistent infections by Pseudomonas aeruginosa are initiated by interaction of a type IV pilus (T4P) with receptors on the mucosal cells of susceptible hosts. Here, we examine the structural changes occurring between the ...
Characterizing the Structure-Function Relationship of Pathological Proteins
Protein structure and dynamics dictate the fundamentals of protein function. For example, a structured protein must change its conformation when interacting with various ligands. In addition, misfolded or intrinsically ...
Characterizing Conformational Dynamics and Catalytic Activity of Enzymes by Hydrogen-Deuterium Exchange Mass Spectrometry
Determining the conformational dynamics of enzymes as they undergo catalysis has been challenging for decades due to the short timescale and limitations of traditional analysis, including techniques such as CPMG 2D NMR and ...