Forer, ArthurXie, Lele2021-02-222021-02-222008-11Cell Motil Cytoskeleton. 2008 Nov;65(11):876-89.0886-1544https://doi.org/10.1002/cm.20309http://hdl.handle.net/10315/38107We added jasplakinolide to anaphase crane-fly spermatocytes and determined its effects on chromosome movement. Previous work showed that actin depolymerizing agents such ascytochalasin D or latrunculin B blocked or slowed chromosome movements; we wanted to compare the effects of jasplakinolide, a compound that stabilizes actin filaments against depolymerization. Jasplakinolide had the same effect on movements of each half-bivalent in a separating pair of half-bivalents, but different half-bivalent pairs in the same cell often responded differently, even when the concentrations of jasplakinolide varied by a factor of two. Jasplakinolide had no effect on about20% of the pairs, but otherwise caused movements to slow, or to stop, or, rarely, to accelerate. When cells were kept in jasplakinolide, stopped pairs eventually resumed movement; slowed pairs did not change their speeds. Confocal microscopy indicated that neither the distributions of spindle actin filaments nor the distributions of spindle microtubules were altered by the jasplakinolide. It is possible that jasplakinolide binds to spindle actin and blocks critical binding sites, but we suggest that jasplakinolide affects anaphase chromosome movement by preventing actin-filament depolymerization that is necessary for anaphase to proceed. Overall, our data indicate that actin is involved in one of the redundant mechanisms cells use to move chromosomes.enWiley This is the peer reviewed version of the following article: [Cell Motil Cytoskeleton. 2008 Nov;65(11):876-89.], which has been published in final form at [ https://doi.org/10.1002/cm.20309]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.Attribution-NoDerivatives 4.0 Internationalchromosome movementcrane fly spermatocytesactinArticlehttps://www.ncbi.nlm.nih.gov/https://onlinelibrary.wiley.com/https://pubmed.ncbi.nlm.nih.gov/18688844/