Slavkovic, SladjanaJohnson, Philip E2022-07-082022-07-082018-06-12Aptamers, 2 (2018) 45-512514-3247http://hdl.handle.net/10315/39531Isothermal titration calorimetry (ITC) is a powerful technique for studying binding interactions. From a single ITC experiment it is possible to quantify the affinity and thermodynamics of a binding event. Here, we outline an experimental approach for performing ITC experiments with a focus on aptamer-small molecule interactions. We also discuss some common problems that can be encountered and how to resolve these issues.enAttribution-NonCommercial-NoDerivatives 4.0 InternationalArticle