Birot, OlivierLemieux, Pierre Ferruccio Marcel2022-12-142022-12-142022-06-092022-12-14http://hdl.handle.net/10315/40629Skeletal muscle angio-adaptation refers to coordinated molecular and cellular events that regulate the expansion (angiogenesis) or regression of the muscle capillary network. The influence of environmental stressors (hypoxia, cold-stress) on skeletal muscle angio-adaptive responses are less understood in comparison to exercise-training or diseased states. This thesis addresses the influence of hypoxia and cold-stress on skeletal muscle angio-adaptation through a literature review and original research, respectively. After reviewing the literature, ambient hypoxia alone may be insufficient in improving muscle capillarization, however ambient hypoxia superimposed with exercise enhances the angiogenic response of exercise-training. In-vitro, the effect of cold-stress with respect to the angiogenic relationship between the myocyte and endothelial cell was investigated. Cold-stress reduced the secretion of angiogenic molecules from the myocyte. Furthermore, cold-stress abrogated endothelial cells proliferation and attenuated VEGFR2 activation. Endothelial cells pre-conditioned to cold-stress and challenged for migration in re-warming conditions however, exhibited an enhanced migratory responsiveness.Author owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.KinesiologyElectronic Thesis or Dissertation2022-12-14Skeletal muscleCapillaryAngiogenesisVEGF-AThrombospondin-1HypoxiaAltitudeExerciseCold-stressHypothermiaColdProteomeMuscle cellMyotubesAngiokineMyokineAngiokine secretionAngio-adaptationMigrationProliferationWestern blotPCRBoyden chamberMyotubeVEGFR2VEGF-R2THBS1THBS-1TSP-1Vascular endothelial growth factor -AVascular endothelial growth factor AC2C12 myotubesC2C12Endothelial cellsEndothelial cellSkeletal muscle endothelial cellSMECmSMECMurine skeletal muscle endothelial cell