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Browsing Biology by Subject "16S rRNA"
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Item Open Access Estimating Broad-Sense Heritability Of The Gut Microbiome In Western Honey Bees (Apis Mellifera)(2025-04-10) Subramanian, Aishwarya; Zayed, AmroThe honey bee gut microbiome, crucial for bee health, may be shaped by both environmental and genetic factors. While differences in microbiomes exist between honey bee subspecies, it is unclear if genetic variation within a population influences this variation. This study estimated the broad-sense heritability of gut microbiome traits to explore patriline effects. We analyzed 500 workers from two colonies using 16S rRNA sequencing for microbiome characterization and genotyped them at 11 microsatellite loci. Results showed that patriline genetics marginally influenced the relative abundance of dominant microbiome taxa (18% to 24%) but did not significantly impact presence/absence of bacterial taxa or alpha and beta diversity. These findings suggest that environmental factors play a more substantial role in shaping the honey bee gut microbiome, indicating that microbiome-based interventions could benefit honey bees across populations.Item Open Access Investigating the Response of the Gut Bacterial Community and Enzyme Activity During the Challenge of Diet Manipulation in the Herbivorous Fish Compostoma Anomalum (Centre Stoneroller) and the Carnivorous Fish Etheostema Caeruleum (Rainbow Darter)(2018-08-27) Dhakal, Pranav; Bucking, CarolEnzymes are biologically important as they are involved in metabolic processes including catabolizing macromolecules for cellular fuel production and maintaining homeostasis. The combined influence of the GIT (gastrointestinal tract) bacterial community and altered diets on the activity of enzymes has been previously postulated but not studied in depth. Therefore, a proper understanding of the contribution of the GIT bacterial community and diet towards the enzyme activity in the GIT and other tissues was required. Hence, in this thesis, I characterized the GIT bacterial communities and enzyme activates in the herbivorous C.anomalum (central stoneroller) and the carnivorous E.caeruleum (rainbow darter) for the first time. Through this thesis, I deduced that the GIT, GIT bacterial community, and GIT enzymes of both fish species each responded distinctly against the challenge of an altered diet and positively benefitted their host in maintaining an overall pinnacle digestive physiology. This response appears confined to the GIT.